A genome-wide association study (GWAS) was conducted on 29 body conformation traits in Chinese Holstein. The experimental population consisted of 445 cattle, each genotyped by the GGP (GeneSeek genomic profiling)-BovineLD V3 SNP chip, which had 26,151 public SNPs. After data cleaning, 20,632 SNPs were retained for GWAS. The phenotypes included 23 categorical body conformation traits measured on 1 to 9 scores and 6 functional body conformation traits measured on 1 to 100 scores. A fixed-effect linear regression model was used to estimate the association effects of SNPs on each of the phenotypes. Genome-wide significant and suggestive thresholds were set up to be 2.42E-06 and 4.85E-05, respectively. The linear statistical model identified to be 79 genome-wide significant (P < 0.05) SNPs on 14 body conformation traits in this Chinese Holstein population. These significant SNPs were mainly located on chromosome 18, 21, 25 and X. Among the significant SNPs, 28 were identified associated with rump angle (RA) and 14 associated with conformation final score (FS). These SNPs may increase our understanding of the underlying biology of body conformation traits.